Ethanol Solutions
Separate Protein Gels Faster
Fisher Bioreagents Buffers
Restriction Enzymes

Value of Choosing Fisher Bioreagents™ OPTIZYME™ Restriction Enzymes

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Purity: OPTIZYME restriction enzymes are produced using high-yielding expression vectors, resulting in a higher level of purity and lower processing time than native restriction enzymes.

Consistency: OPTIZYME restriction enzymes are manufactured in class D clean rooms using recombinant production techniques to yield large lot sizes. The combination of leading production technology with ISO9001 quality and ISO14001 environmental management systems ensures high digestion and ligation efficiency, as well as batch-to-batch reproducibility.

Value Pricing: The use of high-yielding expression vectors and bacterial strains enables Fisher Bioreagents to offer recombinant enzymes with the lowest price/unit charges, allowing our customers to benefit from both substantial savings and improved consistency and quality.

Bioreagent Buffers

Buffers for Every Budget and Scale

  • Economical powders come in various package sizes
  • Concentrated stock solutions provide convenience in one step of easy dilution
  • Ready-to-use solutions offer the biggest time savings of all

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Fisher Bioreagents™ CellPURE™ Biological Buffers

CellPURE Biological Buffers from Fisher Bioreagents are ultrapure zwitterionic buffers that possess both positive and negative charges. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays and other biochemical applications. Manufactured under strict quality control guidelines to ensure performance and reliability

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Advantages

  • Cell culture tested
  • Analyzed for the absence of nucleases and proteases
  • Tested for endotoxin and bioburden levels
  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Minimal permeability to biological membranes

Applications

  • Tissue culture media and maintenance of cell lines
  • Enzyme assays
  • Electrophoresis of RNA
  • Transfection of mammalian cells
  More

Technical Tip

The Water Makes a Difference. CellPURE Biological Buffers are of the highest quality (purity ≥ 99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk for contamination of buffer solutions. Fisher BioReagents provides several grades of high-purity water suitable for the preparation of high-quality buffer solutions.

Choose Your High-Purity Water

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JustPURE "Good" Buffers

Popularly known today as “Good” buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate and bicarbonate.

“Good” buffers approach the “ideal” buffer state by having pKa values at or near physiological pH and minimal interference with biological processes. JustPURE “Good” buffers from Fisher Bioreagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content.

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  • Ultrapure zwitterionic buffers
  • Optimised for research in cellular and molecular biology
  • Low interference with biological reactions

Advantages

  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Chemically and enzymatically stable
  • Minimal permeability to biological membranes
  • Limited effect on biochemical reactions
  • Minimal absorption in spectral range 240 to 700nm

Applications

  • High efficiency transfection of mammalian cells
  • Gel electrophoresis of RNA
  • Protein isolation applications
  • Cell cultures and enzyme assays
  • Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE
  More
Antibiotics and Antimycotics

Optimised for Cell Culture

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In many life science laboratories, the in vitro culturing of bacterial, plant and animal cells is a routine task. High-quality antibiotics and antimycotics from Fisher BioReagents can be used to ensure successful growth of cells by eliminating unwanted bacterial strains and fungi, while maintaining the health and vitality of the desired cells.

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Advantages

  • High quality - antibiotics and antimycotics meet rigorous quality control tests, including verification of potency, purity and solubility using microbiological and chromatographic methods
  • Built for selection - excellent choice of antibiotics for selection of antibiotic resistance genes in both bacterial and mammalian cells
  • High purity and stability - ultrapure antibiotics ensure successful growth of target cells and reproducible results
  • Maximum convenience - antibiotics and antimycotics come in both sterile powder and liquid forms

Applications

  • Plant cell culture
  • Mammalian cell culture
  • Tissue culture
  • Genetic marker selection
Water
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Water plays a fundamental role in Life Science applications and workflows depending upon properties such as water purity, grade, sterility and additives. Fisher Bioreagents' range of water is ideal for many sensitive procedures such as PCR, electrophoresis, DNA sequencing, and buffers for enzymatic analyses.

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