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Thermo Scientific™ GeneJET™ Whole Blood RNA Purification Mini Kit
Designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids
Brand: Thermo Scientific™ K0761
Additional Details : Weight : 3.75120kg
Includes: Lysis Buffer, Wash Buffer WB 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET™ RNA Purification Columns (pre-assembled with collection tubes), Collection tubes (2mL), Collection tubes (1.5mL), Detailed Protocol
Description
The kit utilizes silica based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions or time consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications such as RT-PCR, RT-qPCR, Northern blotting and other RNA based analysis.
Highlights
- Efficient: Up to 1.8μg of total RNA from 500μL of human blood
- Convenient: Spin columns are capped and assembled with collection tubes
- Pure: RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications, validated in cDNA synthesis and quantitative RT-PCR
- Fast and simple: Procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
- Compatible: Suitable for wide variety of samples: human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow and related body fluids
Applications:
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR,RT-qPCR, Northern blotting, nuclease protection assay.
Specifications
| RT-PCR, RT-qPCR, Northern blotting, nuclease protection assay | |
| Lysis Buffer, Wash Buffer WB 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET™ RNA Purification Columns (pre-assembled with collection tubes), Collection tubes (2mL), Collection tubes (1.5mL), Detailed Protocol | |
| 50 preps |