Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit

Each preparation recovers up to 1000 μg of high copy plasmid DNA from 350 mL of recombinant E. coli cultures that can be used in a wide variety of molecular biology procedures such as transfection of sensitive cells (e.g. Huh-7), in vivo transfection, transformation, restriction endonuclease digestion, automated sequencing, and in vitro transcription.

Highlights

• Pure—free of endotoxins plasmid DNA suitable for transfection of sensitive cells
• Efficient—plasmid recovery resulting in high DNA yields
• Fast—isolation of high quality plasmid DNA in less than 60 minutes
• Convenient—spin column format, centrifuge or vacuum protocol

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• Transfection of sensitive cell lines
• FastDigest restriction enzyme digestion
• In vivo transfection
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Includes

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (concentrated)
• Wash Solution II (concentrated)
• RNase A Solution
• Elution Buffer (10 mM Tris-HCl, pH 8.5)
• GeneJET Endo-Free Maxi Purification Columns & Collection Tubes (50 mL, blue-capped)
• GeneJET Endo-Free Maxi Filtration Columns & Collection Tubes (50 mL, white-capped)

Hazardous: No

Quality Control:

• The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
• The kit passes QC requirements if the purified plasmid DNA has an A_260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
• The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.