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SW480 (Human colon adenocarcinoma) Whole cell lysate, Non-denatured; Abnova

Product Code. 16059635
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Quantity:
200 μg
Unit Size:
200µg
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16059635 200 μg 200µg
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Product Code. 16059635 Supplier Abnova Supplier No. L056V2.200ug

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Description

Non-denatured whole cell lysate of SW480 (Human colon adenocarcinoma)

  • Tissue: Colon
  • Host: Human
  • Lysis buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Colon
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Human
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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