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Description
Use these pepsin protein digestion kits in your peptide mapping and peptide quantitation workflows to obtain high quality analytical results.
Use Thermo Scientific™ SMART Digest™ Pepsin Kits in your peptide mapping and peptide quantitation workflows to obtain high quality analytical results. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Pepsin Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.
How SMART Digest Pepsin Kits work
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is often used to generate antibody fragments. Pepsin is used to generate F(ab')2 fragments by removing only that portion of the Fc domain beyond the hinge region. The resulting F(ab')2 fragments are composed of two antibody-binding Fab' fragments connected by disulfides in the hinge region.
Specifications
Specifications
| Description | with Magnetic Bead, Bulk Option (No Collection Plate) |
| Capture | None |
| Cleavage | Hydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid |
| Enzyme | Pepsin |
| Format | Bulk Magnetic |
| For Use With (Application) | Peptide Quantitation, Peptide Mapping |
| Product Line | SMART Digest |
| Type | Digest Pepsin Kit |
| Includes | Bulk Magnetic SMART Digest Resin, SMART Digest Buffer |
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