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Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Devices, 10K MWCO

Perform sample buffer exchange with these 10,000-MW cut-off (MWCO) disposable polypropylene cups with integrated, low-binding membranes.

99.00€ - 756.00€

Specifications

Product Line Slide-A-Lyzer™
MWCO 10 kDa
Membrane Cellulose Membrane, Dialysis
Format MINI Dialysis Device
Purification Target Buffer Exchange, Protein
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Products 6
Product Code Brand Description Product Type Quantity Price Quantity & Availability  
Product Code Brand Description Product Type Quantity Price Quantity & Availability  
10185003
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Thermo Scientific™
69570
MINI Dialysis Device Cup, Microcentrifuge 50 Devices
253.00€
Pack of 50
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10483225
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Thermo Scientific™
69572
MINI Dialysis Device Cup, Microcentrifuge 250 Devices
756.00€
Pack of 250
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11809420
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Thermo Scientific™
69574
MINI Dialysis Device Kit Cup, Dialysis Units, Microcentrifuge Tubes 1 Kit (10 Devices)
99.00€
Pack of 10
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11819420
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Thermo Scientific™
69576
MINI Dialysis Device Kit Cup, Dialysis Devices, Float Buoy 1 Kit (10 Devices)
111.00€
Pack of 10
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11542571
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Thermo Scientific™
88401
MINI Dialysis Device Cup, 15 mL Conical 25 Devices
285.00€
Pack of 25
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11562571
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Thermo Scientific™
88404
MINI Dialysis Device Cup, 50 mL Conical 25 Devices
308.00€
Pack of 25
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Description

Description

Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO, are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules greater than 10 kDa in volumes from 10 μL to 2 mL.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialyzing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

Features of Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO:
Three convenient sizes—dialysis devices for 0.1, 0.5, and 2 mL samples fit standard 1.5-mL micro-centrifuge, 15- and 50-mL conical tubes, respectively
Excellent sample recoveries—low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol—pipette sample into the MINI device and place in tube containing the dialysis buffer; no laborious assembly, device preparation, or expensive equipment are required
100% leak-tested—innovative design does not permit 'wicking' that can occur in home-made devices
Time savings—performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification, and conjugation

These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5, and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4–8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters, and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

The 0.1 mL MINI dialysis devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro-centrifuge tubes. Alternatively, as many as 25 MINI devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple samples inside a large beaker. The 0.5- and 2-mL MINI dialysis devices fit in and are capped by inserting them into the included 15- and 50-mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI dialysis devices, low molecular weight contaminant removal and buffer exchange and desalting can be accomplished within 4–8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume.

Using the 0.1 mL MINI device, 100 μL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes. As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume; temperature and agitation rates; the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes; and the pH, ionic strength, and solvent characteristic of the buffers being used.

Specifications

Specifications

Slide-A-Lyzer™
Cellulose Membrane, Dialysis
Buffer Exchange, Protein
10 kDa
MINI Dialysis Device
Store at room temperature
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