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Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1

For use in NucleoSpin™ Plasmid DNA purification kits for rapid small-scale preparation of high-copy plasmids from E. coli. Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1 without RNase A is a replacement component used in the NucleoSpin™ method's cell lysis process to resuspend the cell pellet.

Brand: Macherey-Nagel™ 740911.1

Product Code. 12756553

100.00€ / 1000mL

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Description

The NucleoSpin™ Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mL E. coli overnight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.

After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing, highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used.

Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, all necessary buffers, RNase A, MN wash plate, MN tube strips, cap strips.

Silica-membrane technology
Yield: up to 20μL sequencing grade plasmid DNA
Elution volume: 75μL to 150μL
Culture volume: 1mL to 5mL
Binding capacity: 25μg
Maximum plasmid size <15kb
No use of organic solvents
NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included

For further information on this product contact your local Customer Services.

Specifications

Buffer	Resuspension Buffer
Content And Storage	Room temperature (18-25°C)
For Use With (Equipment)	NucleoSpin™ Plasmid DNA Purification Kits
Odor	Odorless
Product Type	Resuspension A1 Buffer
RNase	No

Color	Colorless
Density	1g/cm³
For Use With (Application)	For Plasmid DNA Purification
pH	7.5–8.5
Quantity	1000 mL
Form	Solution

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Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1

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