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Gibco™ Residual Protein TrypLE™ ELISA Kit
Description
The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.
TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.
Specifications
Specifications
| Assay Range | 0.78 to 50 ng/mL |
| Assay Sensitivity | 0.032 ng/mL |
| Content And Storage | • Pre-coated 96-well plate • Standard assay buffer concentrate • HRP-conjugated detection antibody • Wash buffer concentrate • Chromogen stop solution • Adhesive plate covers Store at 2–8°C. |
| Conjugate | Horseradish Peroxidase (HRP) |
| Immunoassay Kit Format | Sandwich ELISA Kit |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Interassay CV | 6.20% |
| Intraassay CV | 7.20% |
| Label or Dye | Horseradish Peroxidase (HRP) |
| Protein Aliases | TrypLE, R-Protease |
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Safety and Handling
- TRYPLE ELISA RESIDUAL KIT 1 EA
- Danger
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 1 B
- Skin sensitiser Category 1
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P363-Wash contaminated clothing before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 5-chloro-2-methylisothiazol-3(2H)-one, Phosphoric acid
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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