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DiscoverX Ready-to-Assay™ OX1 Orexin Receptor Frozen Cells

Product Code. 17385319
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Quantity:
2 Vials of Cells
Unit Size:
2 ampoules
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Product Code. Quantity unitSize
17385319 2 Vials of Cells 2 ampoules
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Product Code. 17385319 Supplier DiscoverX Supplier No. HTS175RTA

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Description

The Ready-to-Assay™ OX1 Orexin Receptor Frozen Cells measure HCRTR1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

The Ready-to-Assay™ OX1 Orexin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. orexins) based activation of HCRTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress HCRTR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HCRTR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the cells in a convenient thaw-and-use format.

Specifications

Assay Entry OX1
For Use With (Application) Frozen Cells
Primary Agonist orexins
Quantity 2 Vials of Cells
Target Entry HCRTR1
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