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DiscoverX Ready-to-Assay™ EP2 Prostanoid Receptor Frozen Cells

Product Code. 17385339
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Quantity:
2 Vials of Cells
Unit Size:
2 ampoules
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17385339 2 Vials of Cells 2 ampoules
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Product Code. 17385339 Supplier DiscoverX Supplier No. HTS185RTA

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The Ready-to-Assay™ EP2 Prostanoid Receptor Frozen Cells measure PTGER2 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

The Ready-to-Assay™ EP2 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER2 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the cells in a convenient thaw-and-use format.

Specifications

Assay Entry EP2
For Use With (Application) Frozen Cells
Primary Agonist prostaglandin E2
Quantity 2 Vials of Cells
Target Entry PTGER2
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