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Thermo Scientific Chemicals RBC lysis buffer for mouse
Description
RBC Lysis Buffer has been reported for use in flow cytometric analysis and cell culture. Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Spécification
Spécification
| Quantity | 500mL |
| Format | Liquid |
| Product Type | Cell Lysis Reagent |
| For Use With (Application) | For lysis of mouse red blood cells |
| Content And Storage | 2° to 8°C |
Frequently Asked Questions (FAQs)
We recommend the following procedure:
1. Dilute 1 volume of cell suspension with 10 volumes of RBC lysis buffer for mouse.
2. Vortex for 5 sec and incubate for 10 min at room temperature. If the sample is not transparent red after 10 min, incubate for another 2 min. Stop reaction by adding 20-30 mL of 1X PBS.
3. Centrifuge at 300xg for 10 min at 4 degrees C. Aspirate supernatant. White blood cells are present in the pellet.
4. Repeat the lysis process if red blood cells are still present in the pellet.
5. Resuspend the cell pellet in an appropriate buffer and proceed for further applications.
This buffer is able to lyse mouse RBCs. It contains ammonium chloride that lyses the RBC and leaves the WBCs intact. The resulting WBCs can be used for staining or nucleic acid isolation.
RBC lysis buffer for mouse contains 10 mM Tris-HCl, 150 mM ammonium Chloride at pH 7.2-7.4.
RUO – Research Use Only
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