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Rat heart tissue lysate, Non-denatured; Abnova

Product Code. 16039595
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Quantity:
200 μg
Unit Size:
200µg
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Product Code. 16039595

Brand: Abnova L036W2.200ug

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This item is not returnable. View return policy

Description

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Heart
Description Whole tissue lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Rat
Concentration 5 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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