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Merck Millipore Proteinase K

From Tritirachium album

Brand:  Merck Millipore 1245680500

Additional Details : CAS : 39450-01-6 Weight : 0.00050kg

Product Code. 10036570

  • 362.00€ / 500mg

Estimated Shipment: 23-08-2022
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Description

Description

  • Chromatographically purified lyophilized 30 m Anson-U/mg
  • For biochemistry and molecular biology EC 3.4.21.14
  • Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
  • Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
  • Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
  • Determination of enzyme localization on membranes
  • Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
  • Digestion of proteins from brain tissue samples for prions in Transmissible
  • Spongiform Encephalopathies (TSE) research.
  • Proteinase K is a stable and highly reactive serine protease.
  • Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224).
  • It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature.
  • In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
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