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Description
The protein MW marker is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis, as well as on PVDF, nylon and nitrocellulose membranes. The protein standard produces sharp bands on protein mini gels following staining with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains.Highlights:
Size range – seven proteins spanning 14.4 to 116kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels
Sharp bands – equal intensity upon coomassie or silver staining
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Includes:
Proteins (0.1 to 0.2mg/mL of each) in 62.5mM Tris-HCl (pH 7.0 at 25°C), 1mM EDTA, 2% SDS, 50mM DTT, 30mM NaCl, 1mM NaN3, 0.01% bromophenol blue and 50% glycerol; Component proteins: beta-galactosidase (116kDa), bovine serum albumin (66.2kDa), ovalbumin (45.0kDa), lactate dehydrogenase (35.0kDa), REase Bsp98I (25.0kDa), beta-lactoglobulin (18.4kDa), lysozyme (14.4kDa)
Recommended for:
Accurate protein sizing on SDS-polyacrylamide gels and Western blots
Specifications
| 7 | |
| 14.4 to 116 kDa | |
| 116, 66.2, 45, 35, 25, 18.4, 14.4 kDa | |
| 2 x 1 mL |
| Pierce™ | |
| SDS-PAGE | |
| Unstained | |
| Contents: two vials of 1 mL each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 50 mM DTT, 30 mM NaCl, 0.01% bromophenol blue and 50% glycerol Storage: Upon receipt store at -20°C |