missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. SILAC Isotope Labeling Reagents and Kits
  5. Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits

Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits

Product Code. 11688833 Shop All Thermo Scientific Products
Click to view available options
Product Type:
Protein Quantitation Kit - DMEM
Protein Quantitation Kit - DMEM:F12
Protein Quantitation Kit - RPMI 1640
Unit Size:
1 set
Each
This item is not returnable. View return policy

Product Code. 11688833

Brand: Thermo Scientific™ 88439

Please to purchase this item. Need a web account? Register with us today!

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance
 View alternative products

This item is not returnable. View return policy

Description

Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.

Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Reproducible – eliminates intra-experimental variability caused by differential sample preparation

  • Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling

  • Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others

  • Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation

Recommended for:

Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment

TRUSTED_SUSTAINABILITY

Specifications

Formulation Multi-component kit with heavy and light L-Lysine and DMEM:F12 Media
Kit Contents L-Lysine-2HCl, 50mg, Dialyzed FBS, 2 x 50mL, DMEM:F12 Media for SILAC, 2 x 500mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6, 50mg
Product Type Protein Quantitation Kit - DMEM:F12
Sufficient For One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
Quantity 1 Use Kit
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.