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Description
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents.Highlights:
Size range – six proteins spanning 20 to 120kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels
Prestained – for direct viewing during electrophoresis
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Includes:
Dye-stained proteins in 62.5mM Tris-HCl (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1.5mM NaN3 and 33% glycerol; Component proteins: beta-galactosidase (120kDa), bovine serum albumin (85kDa), ovalbumin (50kDa), cardonic anhydrase (35kDa), beta-lactoglobulin (25kDa), lysozyme (20kDa)
Recommended for:
Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots
Specifications
| Colorimetric | |
| Pierce™ | |
| SDS-PAGE | |
| 1 color: Blue | |
| Storage Buffer: 62.5mM Tris•H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% (w/v) SDS, 1mM DTT, 1.5mM NaN3 and 33% (v/v) glycerol. Upon receipt store at -20°C. Product is shipped with an ice pack. |
| 6 | |
| 20 to 120 kDa | |
| 120, 85, 50, 35, 25, 20 kDa | |
| 500 μL |