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Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit

Product Code. 10454165 Shop All Thermo Scientific Products
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Description

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Thermo Scientific™ Pierce Maleimide-Activated HRP Conjugation Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues
TRUSTED_SUSTAINABILITY

Spécification

Chemical Reactivity Thiol
Quantity 1 Reaction
Reactive Moiety Maleimide
Solubility Water
Content And Storage Sufficient For: 1 enzyme-labeling reaction with up to 5 mg of antibody (IgG)
• Maleimide Activated Horseradish Peroxidase, 5 mg
• Conjugation Buffer (10X), 20 mL
• PBS Pack (makes 500mL), 1 pack
• 2-Mercaptoethylamine-HCl, 6 mg
• SATA, 2 mg
• Dimethylformamide, 1 mL
• Hydroxylamine-HCl, 5 mg
• Polyacrylamide Desalting Column, 10 mL, 1 column

Store at 4°C.
Formulation Multi-component kit with activated HRP
Product Line EZ-Link
Sufficient For 1 Enzyme Labeling Reaction
Label Type Enzyme Labeled
Label or Dye HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.

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