missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Target-Specific Western Blot Kits
  5. Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Product Code. 10046053 Shop All Thermo Scientific Products
Click to view available options
Quantity:
1 L Kit
200 mL Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10046053

Brand: Thermo Scientific™ 35055

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

Highlights:

  • Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods

  • Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues

  • Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment

  • Easy – optimized kits includes all reagents except primary antibody

  • Versatile – kits work with both rabbit and mouse primary antibodies

Includes:

Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2

TRUSTED_SUSTAINABILITY

Specifications

Product Type Western Blot Kit
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce™
Quantity 200 mL Kit
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Mouse, Rabbit
Sufficient For 5 Mini Blots
Content And Storage Sufficient For: 5 mini blots probed with mouse or rabbit primary antibodies
• Fast Western Antibody Diluent, 100 mL
• Fast Western 10X Wash Buffer, 50 mL
• Fast Western Optimized HRP Reagent, 5 mL
• Pierce ECL Detection Reagent 1, 25 mL
• Pierce ECL Detection Reagent 2, 25 mL

Store at 4°C.
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.