missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Brand:  Thermo Scientific™ 32134

134.90 EUR valid until 2024-03-29
Use promo code "21653" to get your promotional price.



Additional Details : CAS : 123-91-1 Weight : 0.25000kg Transport : UN number : 1993 Chem class : 3 Pack group : II

 View more versions of this product

Product Code. 11547910

  • 142.00€ / 25mL
Estimated Shipment: 09-04-2024
to see stock.
Add to basket

Description

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

  • Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

  • Higher sensitivity — detect targets down to the low-picogram level

  • Longer signal duration — sustained light output for as long as five hours

  • More imaging options — X-ray, CCD or laser-based imagers

  • More affordable — high quality and performance at a lower price than other suppliers« similar substrates

Specifications

Specifications

Substrate
Liquid
Nitrocellulose, PVDF
25 mL Kit
2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1μg/mL)
5 hr.
Upon receipt store at 4°C. Product shipped on ice.
Chemiluminescent, Fluorescent
Western Blot
Pierce™
Horseradish Peroxidase
Low picogram to mid-femtogram
250 sq. cm of membrane
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.