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Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

Product Code. 10036813 Shop All Thermo Scientific Products
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20 Reactions
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10036813 20 Reactions 1 set
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Product Code. 10036813 Supplier Thermo Scientific™ Supplier No. 89818
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Description

Biotinylate single-stranded DNA primers for non-radioactive EMSA and other nucleic acid detection methods.

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

  • Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
  • 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
  • Biotin-labeled probes are stable for more than one year
  • 30-minute labeling procedure is fast and efficient

    The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.

    • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage Sufficient For: 20 labeling reactions (each with 5 pmol DNA)
    • 1 mL 5X TdT Reaction Buffer (-20°C)
    • 5 μL Terminal Deoxynucleotidyl Tranferase (-20°C)
    • 1 μL Biotin-11-UTP (≤4°C)
    • 14 μL Unlabeled Control Oligo (-20°C)
    • 4 μL Biotin-Control Oligo (-20°C)
    Format Kit
    Labeling Target DNA
    No. of Reactions 20
    Product Line Pierce
    Product Type DNA Labeling Kit
    Quantity 20 Reactions

    Safety and Handling

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    Product Identifier
    • Pierce Biotin 3' End DNA Labeling Kit
     Signal Word
    • Danger
     Hazard Category
    • Acute toxicity Category 4
    • LONG-TERM AQUATIC HAZARD Chronic 2
    • Carcinogenicity Category 1B
     Hazard Statement
    • H302-Harmful if swallowed.
    • H350-May cause cancer.
    • H411-Toxic to aquatic life with long lasting effects.
     Precautionary Statement
    • P201-Obtain special instructions before use.
    • P273-Avoid release to the environment.
    • P280-Wear protective gloves/protective clothing/eye protection/face protection.
    • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
    • P308+P313-IF exposed or concerned: Get medical advice/attention.
    • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
     Supplemental information
    • MIXTURE LIST-Contain: Cobalt(II) chloride,hexahydrate

    For Research Use Only. Not for use in diagnostic procedures.

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