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Thermo Scientific™ Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

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Quantity:
275 mL
Unit Size:
1 set
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Product Code. 10750985

Brand: Thermo Scientific™ 23250

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270.97 EUR valid until 2025-12-19
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This item is not returnable. View return policy

Description

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

  • Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

  • High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Sufficient For: 250 tube assays with 25 μL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 10 x 20 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL

Store at room temperature
Description Pierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)
Assay BCA Assay
Detection Method Colorimetric
Product Line Pierce™
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
Sufficient For 250 Tube Assays
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 275 mL
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Safety and Handling

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Product Identifier
  • Pierce BCA Protein Assay Kit - Reducing Agent Compatible
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 2
 Hazard Statement
  • H301-Toxic if swallowed.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H317-May cause an allergic skin reaction.
  • H400-Very toxic to aquatic life.
  • H411-Toxic to aquatic life with long lasting effects.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P273-Avoid release to the environment.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P391-Collect spillage.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P405-Store locked up.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : sodium carbonate, ¢2, 2'-Biquinoline!-4,4'-dicarboxylic acid, sodium salt (1:2), Sulfuric acid copp

For Research Use Only. Not for use in diagnostic procedures.

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