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DiscoverX PathHunter™ U2OS LCK Activity Assay

Product Code. 17374379
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Quantity:
2 Vials
Unit Size:
2 ampoules
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17374379 2 Vials 2 ampoules
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Product Code. 17374379 Supplier DiscoverX Supplier No. 931100C3

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Description

The PathHunter™ U2OS LCK Activity Assay measures LCK CTK activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

The PathHunter™ U2OS LCK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting CTKs. This cell line enables assessment of CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by LCK-JAK2 fusion protein. This product uses EFC technology. The included cell line overexpresses PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, LCK. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.

Specifications

Assay Entry SH2 Recruitment
Cell Line U2OS
For Use With (Application) Cell Lines
Primary Agonist Recombinant Human Growth Hormone
Quantity 2 Vials
Target Entry LCK
Accession Number NM_000163
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