Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

Description

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

• Size range – seven proteins and one peptide spanning 3.4 to 100kDa

• Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

• Sharp bands – excellent accuracy for detection in with coomassie or silver stains

• Reference bands – 25kDa band is stronger than the others for easy orientation

• Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN₃, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots