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Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder
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Beschreibung
The Thermo Scientific™ PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.
The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.
Highlights:
- Size range 10 proteins spanning 11 to 250kDa
- Fluorescent detect using 670nm red laser or 700nm channels with fluorescence imagers
- Ready-to-use supplied in a loading buffer for direct loading on gels
- Sharp bands excellent sizing for near-infrared visualization of protein gels and blots
- Reference band 55kDa band has greater intensity for easy orientation
- Quality tested each lot evaluated by SDS-PAGE and Western blotting
Includes:
Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol
Requires:
Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System
Recommended for:
Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers
Spezifikation
Spezifikation
| Content And Storage | Contents: two vials of 250 μL each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol Storage: Upon receipt store at -20°C |
| Detection Method | Colorimetric, NIR Fluorescence (700 nm) |
| Number of Markers | 10 |
| Product Type | Protein Ladder |
| Ready to Load | Yes |
| Size Range | 11 to 250 kDa |
| Stain Type | 1 color: Blue |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels |
| Molecular Weight (g/mol) | 250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa |
| Product Line | PageRuler |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
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