missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. 11974812

OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

Product Code. 11974812
Change view
Click to view available options
Quantity:
1000 U
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
11974812 1000 U Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 11974812 Supplier Fisher BioReagents Supplier No. BP80581
Request Bulk or Custom Format

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

5'...A T G C A^T...3'
3'...T^A C G T A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 – 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NsiI.
  • Compatible Ends: Alw21I, PstI, SdaI, SduI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
    pH 7.4
    For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 1000 U
    Cut Site ATGCA.T
    Product Type NsiI
    Form Liquid
    Product Title
    Select an issue

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.