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OPTIZYME™ NotI, Fisher BioReagents™
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Quantity:
1000 U
300 U
Packungsgröße:
Each
Beschreibung
5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'
Conditions for 100% Activity
- 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
- Incubate at 37°C.
Enzyme Activity in OPTIZYME buffers
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 100%
- Buffer 4: 0 - 20%
- Buffer 5: 20 - 50%
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
- GCGGCCGC
Spezifikation
Spezifikation
| Concentration | 10 U/μL |
| Components | 25 to 50% Water, >50% Glycerin |
| pH | 7.4 |
| For Use With (Application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
| Storage Buffer | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
| Content And Storage | Keep container tightly closed |
| Quantity | 1000 U |
| Cut Site | GC.GGCCGC |
| Product Type | NotI |
| Form | Liquid |
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