missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. OPTIZYME™ NotI, Fisher BioReagents™

OPTIZYME™ NotI, Fisher BioReagents™

Product Code. 11944842
Change view
Click to view available options
Quantity:
1000 U
300 U
Unit Size:
Each
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
11944842 1000 U Each
11934842 300 U Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 11944842 Supplier Fisher BioReagents Supplier No. BP80045
Request Bulk or Custom Format

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'

Conditions for 100% Activity

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 3
Enzyme Activity in OPTIZYME buffers
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 20 - 50%
Storage Buffer:
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
  • GCGGCCGC
  • (Cleavage blocked)
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin
    pH 7.4
    For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
    Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 1000 U
    Cut Site GC.GGCCGC
    Product Type NotI
    Form Liquid
    Product Title
    Select an issue

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.