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OPTIZYME™ HpaII, Fisher BioReagents™
Brand: Fisher Bioreagents BP8032-1
Additional Details : Weight : 0.01000kg
Description
5'...C^C G G...3'
3'...G G C^C...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- CCGG
Specifications
| 10 U/μL | |
| 37°C | |
| 10mM Tris HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| 1000 U | |
| HpaII |
| 25 to 50% Water, >50% Glycerin | |
| >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII | |
| Keep container tightly closed | |
| C.CGG | |
| Liquid |