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OPTIZYME™ DNA Polymerase I, Fisher BioReagents™
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Quantity:
2500 U
500 U
Unit Size:
2500 units
500 units
Description
OPTIZYME™ DNA Pol I is a template-dependent DNA Polymerase that catalyzes 5'->3' DNA synthesis, and exhibits 3'->5'-exonuclease, 5'->3' exonuclease and RNase H activity.
Applications and Features:
- OPTIZYME DNA Polymerase I labels DNA by nick translation in conjunction with DNase I.
- Second-strand cDNA synthesis in conjunction with RNase H.
- Incorporation of modified nucleotides (e.g., biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides).
- Active in buffers for OPTIZYME restriction enzymes and reverse transcriptases.
Supplied with:
10X OPTIZYME DNA Pol I Buffer: 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 10mM DTT
Source: E. Coli cells with a cloned polA gene for E.Coli
Quality Control:
Endodeoxyribonuclease-free
Specifications
Specifications
| Concentration | 10 U/μL |
| Color | Colorless |
| pH | 7.5 |
| For Use With (Application) | DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases |
| Storage Buffer | OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM |
| Content And Storage | -20°C |
| Source | E. coli cells with a cloned polA gene |
| Quantity | 2500 U |
| Product Type | DNA Polymerase I |
| Form | Liquid |
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