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OPTIZYME™ ApaI, Fisher BioReagents™

Product Code. 11924792
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5000 U
Unit Size:
Each
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Product Code. 11924792 Supplier Fisher BioReagents Supplier No. BP80251
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Description

5'...G G G C C^C...3'
3'...C^C C G G G...5'

Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 100%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 20 - 50%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1 mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of ApaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note:
    • Incubation at 30°C results in a 2-fold increase in activity.
    • ApaI is inhibited by salt concentrations above 50 mM.
    • ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain.
    Compatible Ends: BseSI, Eco24I, SduI
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: May overlap - cleavage impaired
    • 5'...GGGCCm5CW GG...3'
  • 3'...CCCGG GWm5CC...5'
  • (Cleavage is impaired)
  • CpG: May overlap - cleavage impaired
    • 5'...GGGCCm5C G...3'
  • 3'...CCCGG Gm5C...5'
  • (Cleavage is impaired)
    • 5'...m5C GGGCCm5C G...3'
  • 3'... Gm5CCCGG Gm5C...5'
  • (Cleavage is impaired)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 5000 U
    Cut Site GGGCC.C
    Product Type Apal
    Form Liquid
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