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OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™
Brand: Fisher Bioreagents BP8097-5
Additional Details : Weight : 0.03000kg
Description
OPTIZYME™ AP is a novel alkaline phosphatase that catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides in 10min.
Applications and Features:
- OPTIZYME™ Alkaline Phosphatase dephosphorylates cloning vector DNA (10min at 37°C)
- Fast thermoinactivation (5min at 75°C)
- Simultaneous digestion and dephosphorylation of vector DNA
- Single protocol for all types of DNA ends
- Nucleotide degradation prior to sequencing PCR products
- Active on nucleotides
- Protein dephosphorylation
- Active in all OPTIZYME restriction enzyme buffers
Supplied with:
10X OPTIZYME AP Buffer: 100mM Tris-HCl (pH 8.0 at 37°C), 50mM MgCl2, 1M KCl, 0.2% (v/v) Triton X-100, 1mg/mL BSA
Source: E. Coli cells with a cloned bacterial alkaline phosphatase gene
Quality Control:
- Endo-, exodeoxyribonuclease and ribonuclease-free
- Functionally tested for dephosphorylation of 5'-termini of overhanging, recessed and blunt DNA
Specifications
| 1 U/μL | |
| 7.4 | |
| OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL | |
| E. coli cells with a cloned bacterial Alkaline Phosphatase gene | |
| Alkaline Phosphatase |
| Colorless | |
| DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En | |
| -20°C | |
| 3000 U | |
| Solid |