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OPTIZYME™ AatII, Fisher BioReagents™

Product Code. 11944802
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Quantity:
300 U
Unit Size:
Each
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Product Code. 11944802 Supplier Fisher BioReagents Supplier No. BP80411
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Description

5'...G A C G T^C...3'
3'...C^T G C A G...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of AatII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note: Activity decreases if reaction buffer pH is not between 7.5 and 8.0 at 25°C.
  • Compatible Ends: TaiI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - blocked
    • GACGTC
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - no effect
    • 5'...TGm6ACGTC(N)4 TGCT...3'
  • 3'...AC TGCAG(N)4m6ACGA...5'
  • (No effect on cleavage)

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 300 U
    Cut Site GACGT.C
    Product Type AatII
    Form Liquid
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