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Macherey-Nagel™ NucleoTrap kit for gel extraction
Description
NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes.
By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water.Kit components: NucleoTrap™ suspension, buffers.
- Silica matrix technology
- Fragment size: 20 bp to 20 kb
- Sample material: 20 to 600 mg agarose gel
- Elution volume: 20 to 50 μL
- Binding capacity: 6 μg/10 μL suspension
- Typical Recovery: 50 to 90%
- Preparation time: 60 min./6 preps
- Format: silica bead suspension
Specifications
Specifications
| Capacity | 0.6 μg/10 μL suspension |
| Content And Storage | 18°C to 25°C |
| Isolation Technology | Silica Matrix Technology |
| Sample Type | Agarose Gel |
| No. of Reactions | 100 Preps |
| Sample Volume | <200 mg |
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