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  5. Macherey-Nagel™ NucleoSpin™ RNA Stool, Mini kit for RNA from stool

Macherey-Nagel™ NucleoSpin™ RNA Stool, Mini kit for RNA from stool

Product Code. 15875244
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No. of Reactions:
10
50
Unit Size:
10 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 15875244

Brand: Macherey-Nagel™ 740130.50

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This item is not returnable. View return policy

Description

Complete kits for quick and efficient isolation of total RNA from a variety of stool specimens using a combination of silica membrane technology and NucleoSpin™ Bead Tubes A (included). Kits available for 10 or 50 preps.

RNA Stool kit enables efficient isolation of high quality RNA from any kind of stool sample. The kit combines NucleoZOL and a specific Lysis Buffer to chemically disrupt stool samples. The containing microbes are then disrupted with a mechanical lysis using the included Bead Tubes Type A.

  • Optimized workflow ensures a fast isolation of high quality RNA, including small RNA
  • Combination of mechanical homogenization and chemical lysis
  • Isolated RNA does not show any PCR inhibition due to Inhibitor Removal Columns
  • Suitable for various human and animal stool samples, fresh or frozen

Specifications

Format Mini Spin Column
Isolation Technology Silica Membrane Technology, NucleoSpin Bead Tubes
Elution Volume 100 μL
Sample Type Animal or Human Stool (Fresh or Frozen)
Efficiency Binding capacity: 200 μg
Final Product Type High integrity RNA
For Use With (Application) Isolation of RNA from stool
Includes Columns with Collection Tubes, NucleoZOL, Bead Tubes Type A, Inihibitor Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), rDNase, buffers
No. of Reactions 50
Purity A260/A280: 1.9 - 2.1
Sample Size 180 - 220 mg
Test Sensitivity RNA integrity number (RIN): > 7.5
Test Time 70 min / 10 preps (preparation time)
Yield 10 - 30 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin RNA Stool (50)
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 2
  • Flammable liquid Category 3
  • Germ cell mutagenicity Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 B
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H226-Flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H302-Harmful if swallowed.
  • H311-Toxic in contact with skin.
  • H314-Causes severe skin burns and eye damage.
  • H319-Causes serious eye irritation.
  • H331-Toxic if inhaled.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
  • H341-Suspected of causing genetic defects.
  • H351-Suspected of causing cancer.
  • H373-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P405-Store locked up.
 Supplemental information
  • MIXTURE LIST-Contains : phenol, guanidium thiocyanate, rDNAse, 1,4-dioxane, ethanol
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