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  5. Macherey-Nagel™ NucleoSpin™ RNA Plant and Fungi, Mini kit for RNA from plant and fungi

Macherey-Nagel™ NucleoSpin™ RNA Plant and Fungi, Mini kit for RNA from plant and fungi

Produktkod. 15715543
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No. of Reactions:
10
50
250
Förpackningsstorlek:
10 reactions
250 reactions
50 reactions
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Produktkod. No. of Reactions unitSize
15715543 50 50 reactions
15705543 10 10 reactions
15865244 250 250 reactions
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Produktkod. 15715543 Leverantör Macherey-Nagel™ Leverantörsnummer 740120.50

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Description

Universal kit designed to isolate high-integrity RNA from challenging plant and fungal samples using silica membrane mini spin columns, for use in a variety of downstream applications. Kits with 10, 50 or 250 preps available.

Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds and the types of organs. Common products for RNA isolation often fails to efficiently purify RNA from challenging plant samples.

  • Tailored protocols for diverse starting materials (e.g. seeds, roots, stems, needles)
  • Convenient handling and efficient isolation of high integrity RNA
  • NucleoSpin RNA Plant and Fungi Filters for lysate clearing included
  • Designed for samples rich in secondary metabolites, sugar, starch or other compounds that interfere with nucleic acid purification methods
  • Can also be used for standard samples, such as Arabidopsis leaves
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

Format Mini Spin Column
Isolation Technology Silica Membrane
Elution Volume 50 μL
Sample Type Plant or Fungal Material (Seeds, Roots, Stems, Needles)
Efficiency Binding capacity: 200 μg
Final Product Type High integrity RNA
For Use With (Application) Isolation of RNA from plant and fungal samples
Includes Filters, Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers, RNase free water
No. of Reactions 50
Purity A260/A280: 1.9 - 2.1
Sample Size <500 mg
Test Sensitivity RNA integrity number (RIN): 7 - 9
Test Time 25 min / 6 preps (preparation time)
Yield 20 - 70 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin RNA Plant and Fungi
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash with water thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
 Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride, ethylendinitrilo tetraacetic acid (EDTA)
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