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  5. Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification

Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification

Product Code. 15880968
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Quantity:
250 Columns
50 Columns
Unit Size:
Pack of 250
Pack of 50
This item is not returnable. View return policy

Product Code. 15880968

Brand: Macherey-Nagel™ 740955.50S

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This item is not returnable. View return policy

Description

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

NucleoSpin™ RNA

  • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
  • NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
  • Up to 70 μg ready to use RNA
  • Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
  • RNA isolation from cultured cells, tissue (standard protocol):
    • RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
    • RNA from ≤ 100 μL biological fluids
    • RNA clean-up from reaction mixtures
    • RNA from samples stored in RNAlater™
    • RNA from saliva samples collected with Oragene™ RNA (Genotek)
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Tekniske data

Color Blue band
Content And Storage rDNase at 4 °C; Other components at room temperature (18°C to 25 °C)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Elution Volume 40 to 120 μL
Sample Type Cultured Bacterial or Yeast Cells, Human or Animal Tissue
Efficiency Binding capacity: 200 μg
For Use With (Equipment) NucleoSpin RNA Kits
For Use With (Application) Isolation of RNA from cells and tissue
Includes Columns only
Quantity 50 Columns
Sample Size < 30 mg tissue, 5 x 106 cultured cells
Test Time 35 min/6 preps
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Sikkerhed og håndtering

Produktidentifikator
  • NucleoSpin RNA
 Supplerende oplysninger
  • MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu
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