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  6. Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Product Code. 15503636
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No. of Samples:
50
250
Unit Size:
250 reactions
50 reactions
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Product Code. No. of Samples unitSize
15503636 50 50 reactions
15593626 250 250 reactions
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Product Code. 15503636 Supplier Macherey-Nagel™ Supplier No. 740490.50

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Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via manual, vacuum or centrifugation processing
  • Ultra-fast procedure using mini spin columns simplifies the work-flow
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Spécification

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format Mini Spin Column
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
Efficiency Binding capacity: 35 μg
Elution Volume Elution Volume: 30 - 50 μL
Final Product Type Transfection-grade plasmid DNA
For Use With (Equipment) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
For Use With (Application) Rapid purification of plasmids for transfection
Includes NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
No. of Samples 50
Purity A260/A280: 1.80 - 1.85
Sample Volume Up to 5 mL
Size < 15 kbp
Test Time 14 min/6 preps
Yield 15 - 30 μg
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