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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

Brand:  Macherey-Nagel™ 740609.240C


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Product Code. 15116456

  • 540.00€ / 240 preps
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Description

Description

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Specifications

Specifications

pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Silica Membrane Technology
Elution volume: 30–100μL
Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60)
1 Kit
<400μL (PCR reaction mixture)
<400mg (TAE / TBE agarose gel)
10 min/6 preps
Mini Spin Column
Typical recovery: 70–95%
QIAcube™ compatible
TAE or TBE agarose gels, PCR
240 Preps
A260/A280: 1.8–1.9
50 bp to ∼20 kbp
Up to ∼15μg DNA
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H302-Harmful if swallowed.
  • H412-Harmful to aquatic life with long lasting effects.

Supplemental information
  • MIXTURE LIST-Contains : guanidinium thiocyanate

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