Learn More
Macherey-Nagel™ NucleoSpin™ 8 Plasmid, 8-well kit for plasmid DNA
Description
NucleoSpin™ 8 Pasmid kits enable variable-throughput isolation of sequencing-grade plasmid DNA from E. coli. The flexible 8-well strip format allows for automation on common liquid handling instruments or for manual processing without time-consuming centrifugation.
Up to 8 samples per strip can be parallel-processed simultaneously, typically within less than 45 minutes. Uses include isolation of high-copy plasmids, purification of cosmids and reliable downstream applications, such as cloning, sequencing, PCR, transformation, and restriction analysis.
- Time-saving parallel isolation of plasmid DNA using silica-membrane technology
- Processing of up to 5 mL bacterial culture, with a yield up to 6 μg/mL
- 8-well filter strip for convenient filtration of bacterial lysates
- Suitable for manual or automated processing under vacuum
- Innovative MN Wash Plate minimizes risk of cross-contamination
Specifications
Specifications
| Format | 8-well Strip |
| Sample Type | E. coli Culture |
| Efficiency | Binding capacity: 20μg |
| Elution Volume | Elution volume: 75–150μL |
| Final Product Type | Sequencing grade plasmid DNA |
| For Use With (Equipment) | Liquid handling instruments |
| For Use With (Application) | Manual and automated processing under vacuum |
| Includes | NucleoSpin Plasmid Filter Strips (60), NucleoSpin Plasmid Binding Strips (60), MN Wash Plate (5), Culture plate with Gas-permeable Foil (5), Rack of Tube Strips (5), RNase A (60mg), Resuspension Buffer A1 (150mL), Lysis Buffer A2 (150mL), Neutralization Buffer A3 (200mL), Wash Buffer AW (500mL), Wash Buffer A4 Concentrate (4 x 100mL), Elution Buffer AE (125mL) |
| No. of Reactions | 60 x 8 Preps |
| Product Type | NucleoSpin™ 8 Plasmid Kit |
| Show More |
Safety and Handling
- Danger
- Flammable liquid Category 3
- Acute toxicity Category 4
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
- Specific target organ toxicity Category 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt; guanidine hydrochloride; 2-
Your input is important to us. Please complete this form to provide feedback related to the content on this product.