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Macherey-Nagel™ NucleoBond™ Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA
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No. of Reactions:
10
50
Packungsgröße:
10 reactions
50 reactions
Beschreibung
Features
- Endotoxin-free
- Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
- Patented endotoxin removal by additional washing step – no extra ice incubation necessary
- Optimized column design – Midi prep in 45 min.
- Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
- Improved silica material – typical yields of 400μg (Midi)
- High-copy plasmids from E. coli
- Low-copy plasmids from E. coli (increased buffer volumes required)
Spezifikation
Spezifikation
| Buffer | Yes |
| Format | Midi Gravity-flow Column |
| Isolation Technology | Anion Exchange Chromatography Technology |
| No. of Reactions | 50 |
| RNase | Yes |
| Sample Volume | <200 mL (high copy), <400 mL (low copy) |
| Size | <300 kbp |
| Yield | 400μg |
Sicherheit und Handhabung
Signalwort
- Danger
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- H226-Flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- MIXTURE LIST-Contains : 2-propanol/ethanol(diluted < 20 %)/sodium hydroxide solution(diluted < 2 %),dodecyl sulfate,sodium s
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