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  5. Macherey-Nagel™ NucleoBond™ Xtra Maxi kit for transfection-grade plasmid DNA

Macherey-Nagel™ NucleoBond™ Xtra Maxi kit for transfection-grade plasmid DNA

Product Code. p-8013728
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No. of Reactions:
2
10
50
100
Unit Size:
0.1kg
100 preps
2 reactions
50 preps
4 product options available for selection
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Product Code. No. of Reactions unitSize
11902312 100 100 preps
12792603 10 0.1kg
12748412 50 50 preps
11985442 2 2 reactions
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This item is not returnable. View return policy
Product Code. 11902312 Supplier Macherey-Nagel™ Supplier No. 740414.100

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This item is not returnable. View return policy

Descripción

Purify transfection-grade plasmid DNA with high yield. The NucleoBond™ Xtra Maxi Kit represents the new generation of anion exchanger. The optimized design results in high yield and ultra-fast processing.

Features

  • Optimized column design—Silica resin packed between filters, with prep in just 75 minutes
  • Column filter included—High filter flow rates, parallel lysate clearing, and loading onto the column
  • Improved silica material—Typical yields of 1,000μg and can be used over a wide pH range
  • Proven anion-exchange technology—Transfection-grade plasmid DNA
  • Kits contain: NucleoBond™ Xtra Maxi columns and filter, RNase A, buffers (RES, LYS, NEU, EQU, WASH, ELU) and plastic washers

Applications

  • High-copy plasmids from E. coli
  • Low-copy plasmids from E. coli (increased buffer volumes required)

Especificaciones

Buffer Yes
Format Maxi Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
No. of Reactions 100
Purity A260/A280:1.80–1.95
RNase Yes
Sample Volume <600 mL (high copy), <1200 mL (low copy)
Size <300 kbp
Test Time 75 min./prep
Yield 1000μg

Seguridad y manipulación

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Palabra de advertencia
  • Danger
 Clasificación
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
 Indicaciones de peligro
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Consejos de prudencia
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Otros peligros
  • MIXTURE LIST-Contain: 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <
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