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  5. Macherey-Nagel™ NucleoBond™ Xtra Midi Columns for transfection-grade plasmid DNA

Macherey-Nagel™ NucleoBond™ Xtra Midi Columns for transfection-grade plasmid DNA

Product Code. 15829097
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For Use With (Equipment):
NucleoBond Xtra Maxi
NucleoBond Xtra Midi
Unit Size:
Pack of 10
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Product Code. For Use With (Equipment) unitSize
15829097 NucleoBond Xtra Midi Pack of 10
15749999 NucleoBond Xtra Maxi Pack of 10
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Product Code. 15829097 Supplier Macherey-Nagel™ Supplier No. 740410.10S

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Description

Polypropylene gravity flow columns with inserted column filters for use with NucleoBond Xtra Midi or Maxi kits for ultra-fast isolation of transfection-grade plasmid DNA with high yield.

NucleoBond™ Xtra—The new generation of anion exchanger for ultra-fast transfection-grade plasmid DNA

  • Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
  • Available in Midi and Maxi sizes – typical yields of 400 μg (Midi) and 1000 μg (Maxi)
  • Polypropylene columns suitable for use with organic solvents and buffers with denaturing agents
  • Column filter included for high filter flow rates, parallel lysate clearing, and loading onto the column
    • Depth filters avoid time-consuming centrifugation for lysate clearing
    • Complete removal of precipitate even with large lysate volumes without clogging
    • Avoids shearing of large DNA constructs (such as PACs or BACs)

Specifications

Content And Storage Room temperature (18°C to 25°C)
Isolation Technology Anion Exchange Chromatography Technology
Final Product Type Transfection grade DNA
For Use With (Equipment) NucleoBond Xtra Midi
For Use With (Application) Plasmid DNA purification
Includes NucleoBond Xtra Column Filters
Product Type Gravity Flow Column
Quantity 10 Pk.
Purity A260/A280: 1.80 - 1.95
Sample Volume < 200 mL (high copy), < 400 mL (low copy)
Size < 300 kbp
Test Time 30 min.
Yield 400 μg
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Safety and Handling

Product Identifier
  • NucleoBond Xtra Midi
 Supplemental information
  • MIXTURE LIST-Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <
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