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  5. Macherey-Nagel™ NucleoBond™ PC 500, Maxi Kit for Transfection-grade Plasmid DNA

Macherey-Nagel™ NucleoBond™ PC 500, Maxi Kit for Transfection-grade Plasmid DNA

Product Code. 12840731
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No. of Reactions:
10 Preps
100 Preps
25 Preps
50 Preps
Unit Size:
10 reactions
100 reactions
25 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 12840731

Brand: Macherey-Nagel™ 740574

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This item is not returnable. View return policy

Description

Recover and purify plasmid DNA without degredation. NucleoBond™ PC 500 Columns use a silica-based anion exchange technology to concentrate and purify transfection-grade DNA.

Features

  • The classic anion exchanger for purification of transfection-grade plasmid DNA
  • Fast and easy clarification of lysates with NucleoBond™ Folded Filters, no centrifugation required, no shearing forces
  • NucleoBond™ Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation
  • Proven anion-exchange technology – transfection-grade plasmid DNA
  • Separate binding columns of NucleoBond™ PC kits available as NucleoBond™ AX columns

Applications:

  • High-copy plasmids from E. coli
  • Low-copy plasmids from E. coli (increased buffer volumes required)

Specifications

Format Maxi
Isolation Technology Anion Exchange Chromatography Technology
Final Product Type Columns PC 500
For Use With (Application) High-copy/low-copy plasmids from E. coli
Includes AX 500 Column, Buffers, RNase A, NucleoBond Folded Filters XL
No. of Reactions 10 Preps
Product Type Anion exchange column
Sample Volume 30–150mL (HC)
100–500mL (LC)
Size <300 kbp
Test Time Prep time: 80–90 min./4 preps
Yield 400 to 500μg
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Safety and Handling

compliance-icons compliance-icons compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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