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Macherey-Nagel™ NucleoBond™ BAC 100 Kit

Suitable for constructs up to 300 kbp. Macherey-Nagel™ NucleoBond™ BAC 100 Kit is an anion exchanger for purification of P1, BACs, PACs, and other large constructs from E. coli for use in downstream applications, including cloning, PCR, restriction analysis, sequencing and transfection.

Brand:  Macherey-Nagel™ 740579


Product Code. 12768482

  • 281.00€ / 10 reactions
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Description

Description

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. The BAC 100 kit is designed for the isolation of low-copy plasmids from large bacterial cultures, and can accommodate constructs up to 300 kpb.

  • Folded Filters are included for gentle lysate clarification without shearing of large constructs
  • Contains sufficient buffer to perform 10 maxi preps, with typical yields of 10–100μg of plasmid DNA from E. coli culture
  • Columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours
Specifications

Specifications

Yes
Anion Exchange Chromatography Technology
Purification of P1, BACs, PACs, and other large constructs
10 Preps
1 Kit
100—500mL
120 min/2–4 preps
Gravity-flow Column
E. coli Culture
Resuspension Buffer S1 (250mL), Lysis Buffer S2 (250mL), Neutralization Buffer S3 (250mL), Equilibration Buffer N2 (70mL), Wash Buffer N3 (2 x 250mL), Elution Buffer N5 (200mL), RNase A lyophilized (25mg), NucleoBond BAC 100 Columns (10), NucleoBond Folded Filters XL (10), Plastic Washers (5)
Column BAC 100
A260/A280: 1.80–1.95
<300kbp
10–100μg
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Safety and Handling

Safety and Handling

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Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

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