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Macherey-Nagel™ NucleoBond™ BAC 100 kit for large construct plasmid DNA

Product Code. 12768482
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Quantity:
1 Kit
Unit Size:
10 reactions
This item is not returnable. View return policy

Product Code. 12768482

Brand: Macherey-Nagel™ 740579

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This item is not returnable. View return policy

Description

1st generation anion exchange technology for large construct plasmid DNA

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. The BAC 100 kit is designed for the isolation of low-copy plasmids from large bacterial cultures, and can accommodate constructs up to 300 kpb.

  • Folded Filters are included for gentle lysate clarification without shearing of large constructs
  • Contains sufficient buffer to perform 10 maxi preps, with typical yields of 10–100μg of plasmid DNA from E. coli culture
  • Columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours

Specifications

Buffer Yes
Format Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
Sample Type E. coli Culture
For Use With (Application) Purification of P1, BACs, PACs, and other large constructs
Includes Resuspension Buffer S1 (250mL), Lysis Buffer S2 (250mL), Neutralization Buffer S3 (250mL), Equilibration Buffer N2 (70mL), Wash Buffer N3 (2 x 250mL), Elution Buffer N5 (200mL), RNase A lyophilized (25mg), NucleoBond BAC 100 Columns (10), NucleoBond Folded Filters XL (10), Plastic Washers (5)
No. of Reactions 10 Preps
Product Type Column BAC 100
Quantity 1 Kit
Purity A260/A280: 1.80–1.95
Sample Volume 100—500mL
Size <300 kbp
Test Time 120 min/2–4 preps
Yield 10–100μg
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
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