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Novus Biologicals™ pCL-Eco Retrovirus Packaging Vector

Applications: Retroviral Production

Brand:  Novus Biologicals™ NBP2-29540

Encompass

Product Code. 15776817

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Description

Description

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

  • Species: Human, Mouse, Rat
  • Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster)
  • For large scale production, this plasmid may be amplified on LB plates containing 50µg/mL ampicillin
  • Buffer: 10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Specifications

Specifications

10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Store at -20°C. Avoid freeze-thaw cycles.
10 μg
pCL-Eco Retrovirus Packaging Vector
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