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Thermo Scientific™ North2South™ Chemiluminescent Substrate for HRP

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Quantity:
100 mL Kit
Unit Size:
100mL
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Product Code. 11876704

Brand: Thermo Scientific™ 17295

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Description

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Sufficient For: 1000 cm2 of membrane (10 mini-gel blots)
• Luminol/Enhancer Solution, 50 mL
• Stable Peroxide Solution, 50 mL

Store at 4°C.
Detection Method Chromogenic
Product Line North2South
Product Type Substrate
Quantity 100 mL Kit
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