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Thermo Scientific™ North2South™ Biotin Random Prime DNA Labeling Kit

Produce biotinylated DNA templates for DNA hybridization and detection methods using random heptanucleotides, Klenow fragment and biotin-nucleotides.

Brand:  Thermo Scientific™ 17075

Additional Details : Weight : 4.08230kg

Product Code. 10229423

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance


Description

Description

Thermo Scientific™ North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.

Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA polymerase. Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations. The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP).

Highlights:

  • Ultimate sensitivity - labels with biotinylated dUTP, maximizing streptavidin-HRP binding and increasing sensitivity
  • Works with as little as 100ng starting DNA template
  • Exonuclease-free Klenow fragment produces higher yields
  • Each reaction yields probe sufficient for approximately three (10 x 10cm) blots
Specifications

Specifications

Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)
• Heptanucleotide Mix (5X), 100 μL
• Deoxynucleotide Mix (5X), 80 μL
• Reaction Buffer (10X), 50 μL
• DNA Polymerase Klenow Fragment (5U/μL), 10 μL
• Control DNA (250 ng/μL), 5 μL
• Ammonium Acetate (5M), 1 mL
• EDTA (0.5M, pH 8.0), 1 mL
• Nuclease-free Water, 1 mL
• Biotin-11-dUTP, 20 μL

Store all components at -20°C.
DNA
North2South™
10 Reactions
Kit
10
DNA Labeling Kit
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