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Invitrogen™ Mouse MCP-1 ELISA Kits

Product Code. 10107783
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Quantity:
1 Plate Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10107783

Brand: Invitrogen™ EMMCP1

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Description

Measure levels of mouse monocyte chemoattractant protein-1 (MCP1) in mouse serum, plasma and cell culture supernatant samples using 96-well plates.

Assay MCP-1 (mouse monocyte chemoattractant protein-1) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific™ Mouse MCP-1 ELISA Kits.

These mouse MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 16 to 1000pg/mL

  • Sensitivity: < 4pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1α, RANTES or TNFα, or human or rat MCP-1

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 16 to 1000pg/mL
Assay Sensitivity <4pg/mL
Description Mouse MCP-1 ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Specificity No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1a, RANTES or TNFa, or human or rat MCP-1
Sufficient For 96 microplate wells
Kit Contents TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75 μL, Stop Solution, 13mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, HRP Dilution Buffer, 13mL, Lyophilized Recombinant Mouse MCP-1 Standard, 2 vials, Sample Diluent, 12mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 1 Plate Kit
Sample Volume 50 μL
Target Natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
Total Assay Time 3 hr.
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