missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Proteintech™ Mouse Leptin ELISA Kit
KE10048 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse Leptin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Leptin.
Brand: Proteintech™ KE10048-96T
Additional Details : Weight : 0.15000kg
Description
- The assay recognizes mouse Leptin. An antibody specific for mouse Leptin has been pre-coated onto the microwells.
- The mouse Leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Leptin is added to detect the captured mouse Leptin protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance.
- Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions.
- Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.
Specifications
| BC038162 | |
| 0.4 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 62.5 to 4000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 16846 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P41160 |