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Invitrogen™ Mouse IL-5 ELISA Kits

Product Code. 10571394
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Quantity:
1 Plate Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10571394

Brand: Invitrogen™ EMIL5

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Description

Measure levels of mouse interleukin-5 in mouse serum and culture supernatant samples using 96-well plates.

Assay IL-5 (mouse interleukin-5) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-5 ELISA Kits.

These mouse IL-5 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-5 (IL-5) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-5 antibody pairs and IL-5 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-5 (IL-5)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 20 to 320pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNγ, GM-CSF, TNFα, rat IL-5, rabbit IL-5 or human IL-5

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 20 to 320pg/mL
Assay Sensitivity <5pg/mL
Description Mouse IL-5 ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Serum
Specificity No significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNg, GM-CSF, TNFa, rat IL-5, rabbit IL-5 or human IL-5
Sufficient For 96 microplate wells
Kit Contents TMB Substrate Solution, 13mL, Adhesive Plate Covers, 6 ea, Stop Solution, 13mL, Anti-Mouse IL-5 Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL, Anti-Mouse IL-5 HRP Conjugate Reagent, 12mL, Lyophilized Recombinant Mouse IL-5 Standard, 2 vials, Plate Reagent, 8mL, Standard Diluent, 12mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 1 Plate Kit
Target Natural and recombinant mouse interleukin-5 (IL-5)
Total Assay Time 3.5 hr.
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