missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Nucleic Acid Labeling and Detection
  5. 10780910

Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Product Code. 10780910 Shop All Thermo Scientific Products
Click to view available options
Quantity:
100 reactions
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10780910

Brand: Thermo Scientific™ 20158

Please to purchase this item. Need a web account? Register with us today!

715.14 EUR valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy

Description

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Highlights:

  • Sensitive – chemiluminescent detection comparable to radioactive detection

  • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

  • Flexible – compatible with RNA probes biotinylated by different methods

  • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

  • Non-radioactive – eliminate waste concerns from radioactive RNA probes

TRUSTED_SUSTAINABILITY

Specifications

Assay EMSA Assay
Content And Storage Sufficient For: 100 binding reactions and detection on 1000 cm2 of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C)
• 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C)
• 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C)
• 10 mg/mL tRNA, 100 μL (store at -20°C)
• 10X REMSA Binding Buffer, 1 mL (store at -20°C)
• 50% Glycerol, 500 μL (store at -20°C)
• 2M KCl, 500 μL (store at -20°C)
• 1M MgCl2, 500 μL (store at -20°C)
• DTT, lyophilized (store at -20°C)
• Nuclease-Free Water, 1.5 mL (store at -20°C)
• 5X REMSA Loading Buffer, 1 mL (store at -20°C)
• Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C)
• Luminol/Enhancer Solution, 80 mL (store at 4°C)
• Stable Peroxide Solution, 80 mL (store at 4°C)
• Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C)
• 4X Wash Buffer, 500 mL (store at 4°C)
• Substrate Equilibration Buffer, 500 mL (store at 4°C)
Detection Method Chemiluminescence
Format Kit
Sample Type Protein
For Use With (Equipment) myECL™ Imager, X-Ray Film
No. of Reactions 100 reactions
Product Line LightShift™
Product Type LightShift Chemiluminescent RNA EMSA Kit
Quantity 100 reactions
Target Specificity Not Target-Specific
Technique Enhanced Chemiluminescence, Membrane Blot, Gel Shift
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.